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Fig. 9 | BMC Plant Biology

Fig. 9

From: A cytosolic NAD+-dependent GPDH from maize (ZmGPDH1) is involved in conferring salt and osmotic stress tolerance

Fig. 9

Overexpression of ZmGPDH1 promotes the stomatal closure under salt and osmotic stresses. a The analysis of water loss rate (WLR) of the WT, atgpdhc2, COM-1 and OE-1. b For stomatal closure assays, the abaxial epidermis of rosette leaves were incubated in the light for 1 h to induce the stomatal opening and then treated with water (control), 300 mM mannitol, 150 mM NaCl and 20 μM ABA for 3 h. Bars = 25 μm. c Stomatal aperture was investigated by measuring the length and width of guard cells. Asterisks indicated significant differences from the WT, as determined by Student′s t-test (*P < 0.05, ** P < 0.01)

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