Fig. 8

Overexpression of ZmGPDH1 alleviates the stress-induced membrane injury and cell death. a The Evan′s Blue staining in 3-week-old WT, atgpdhc2, COM-1, COM-2, OE-1 and OE-2 lines irrigated with water (control), 400 mM mannitol and 200 mM NaCl for 2 h. Bars = 50 mm. b The propidium iodide (PI) fluorescence staining in root tips of 7-day-old plants treated on either half-strength MS plates (control), plates with 300 mM mannitol or 150 mM NaCl for 12 h. The images were obtained by cofocal microscope. Bars = 100 μm. TBARS contents (c) and the relative electrolyte leakage (d) of the six lines after 6 days of water (control), 200 mM NaCl or 400 mM mannitol treatments. Asterisks indicated significant differences from the WT by Student′s t-test (*P < 0.05, ** P < 0.01)