Fig. 7
Overexpression of ZmGPDH1 enhances the ROS scavenging capacity under salt and osmotic stresses. Photographs showing representative (a) NBT and (b) 3,3-diaminobenzidine (DAB) staining of 3-week-old WT, atgpdhc2, COM-1, COM-2, OE-1 and OE-2 plants treated with water (control), 200 mM NaCl and 400 mM mannitol for 2 h. Bars = 50 mm. O2.- (c) and H2O2 (d) levels in the six lines after 6 days of water (control), 200 mM NaCl or 400 mM mannitol treatment. Enzyme activity of (e) superoxide dismutase (SOD), (f) catalase (CAT) and (g) ascorbate peroxidase (APX) in the six lines treated as above. Asterisks indicated significant differences from the WT by Student′s t-test (*P < 0.05, ** P < 0.01)