Fig. 2
From: Characterization and functional biology of the soybean aleurone layer
Two-dimensional isoelectric focusing/sodium dodecylsulphate polyacrylamide gel electrophoresis (IEF/SDS-PAGE) analysis of aleurone and mature cotyledon tissue. a) Total soluble protein from mature (150 mg) cotyledon tissue. b) Total soluble protein from aleurone tissue. c) 2-Dimensional SDS-PAGE of aleurone proteome showing 93 proteins isolated and subsequently identified by MS/MS analysis. Spot identification numbers correspond to protein identification numbers listed in Additional file 1: Table S1. d) Classification of proteins identified to be present in the aleurone layer of maturing soybean seed. Of the protein spots analyzed for identification, 44% were confidently assayed to a known protein and these proteins were further categorized by their cellular role as involved in metabolism, storage, structural, carbohydrate, oxidative stress, defense or other. Visual comparisons and aleurone protein identifications show the similarity in protein composition of the aleurone to the cotyledonary tissue in soybean