Fig. 3

Map-based cloning of PMM1. a Linkage map of PMM1 on chromosome 4. The number of recombinants between the molecular marker and PMM1 is indicated. b Location of PMM1. c Gene structure of PMM1. The black boxes represent the exons and lines between the boxes represent introns. The deletion fragment in pmm1–1 is shown. d Genotyping of ZH11 and pmm1–1 by PCR analysis. e Transcript levels of PMM1 in WT and pmm1–1 determined by qRT-PCR. Rice UBIQIUTIN5 was used as an internal control. Data are presented as means ± SE (n = 3). Significant at **P < 0.01