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Fig. 4 | BMC Plant Biology

Fig. 4

From: Proline synthesis in developing microspores is required for pollen development and fertility

Fig. 4

Histochemical localization of GUS activity in anthers of p17340:GUS and pLtp12:GUS transgenic Arabidopsis plants. Inflorescences of p17340:GUS (a-e) and pLtp12:GUS (f-j) transgenic plants were infiltrated with X-Gluc solution, stained overnight, fixed and cleared for microscopic analysis. a, f Whole-mount inflorescences with buds and flowers at various developmental stages showing GUS activity exclusively in anthers. b-e and g-j Transverse sections of anthers at different developmental stages: b, g Stage 8 with pre-meiotic microspore mother cells; c, h Stage 9 with microspore tetrads. A strong GUS activity is visible in the tapetum of pLtp12:GUS (H); d, i Stage 10 with beginning of p17340:GUS expression in developing pollen (d) and residual GUS activity in the degenerating tapetum of anthers extressing the pLtp12:GUS construct (i); e, j Stage 12 with fully developed pollen with strong GUS activity induced by p17340:GUS expression (e). No GUS activity was detected in stage 12 anthers of pLtp12:GUS transgenic plants (j). Bars = 20 μm

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