Fig. 3

Clustal omega alignment of deduced amino acid sequence of Narcissus pseudonarcissus norbelladine synthase (NpNBS) with norcoclaurine synthase (NCS) amino acid sequences obtained from different species: Eschscholzia californica EcNCS1, Thalictrum flavum TfNCS1, Papaver somniferum PsNCS1, and Coptis japonica CjNCS2 (formely CjPR10A). Grey boxes corresponding to Tyr, Lys, Asp and Glu residues forms the NpNBS catalytic residue. The black bar depicts a glycine-rich P-loop domain conserved in NCS and NpNBS protein. The fully conserved residues are marked with an asterisks (*). The positions with conservation between amino acid residues of similar properties are marked with a colon (:) and the positions with conservations between amino acids residues of weakly similar properties are marked with a period (.). Numbers on the right indicate the residues position in the sequence