Fig. 3

Molecular phylogenetic analysis of the deduced amino acid of GmCHS. The deduced amino acid sequences of the GmCHSs from soybean were aligned with characterized CHS and CHS-like proteins from other plant species and the evolutionary tree was generated using the Neighbor-Joining method in MEGA7 [45]. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test is shown next to the branches. Scale bar indicates branch length representing residue substitution per site. GmCHS are indicated in bold. At, Arabidopsis thaliana; Os, Oryza sativa; Ms., Medicago sativa; Mt., Medicago truncatula; Md, Malus domestica; Pv, Phaseolus vulgaris; Vr, Vitis riparia; Vv, Vitis vinifera; Ah, Arachis hypogaea; Pr,Pinu sradiata; Hp, Hypericum perforatum; Ns, Nicotiana sylvestris; Hv, Hordeum vulgare; Ta,Triticum aestivum; Ata, Aegilops tauschii; AhCHL, Arabidopsis halleri; Pn, Psilotum nudum; Nb, Nicotiana benthamiana; Nt, Nicotiana tobaccum; Zm, Zea mays