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Fig. 3 | BMC Plant Biology

Fig. 3

From: The walnut transcription factor JrGRAS2 contributes to high temperature stress tolerance involving in Dof transcriptional regulation and HSP protein expression

Fig. 3

Transient co-expression analysis of the interactions obtaining from the yeast one-hybrid assays. a, Diagram of the reporters and effecters. Triple tandem copies of the DOFCOREZM motif were fused with the 35S CaMV-46 minimal promoter and cloned into pCAMBIA1301 for driving the GUS gene which used as the reporter construct. The CDS of JrDof3 was cloned into pROKII under the control of the 35S promoter and used as the effecter construct. b, The GUS activities of the transformed tobacco seedlings were determined. pCAM1301 + pROKII-JrDof3, positive control, pCAMA1301 vector transformed with JrDof3; pROKII + pROKII-JrDof3, negative control, pROKII vector transformed with JrDof3. pCAM1301-DOF + pROKII-JrDof3, pCAM1301-DOF-M + pROKII-JrDof3, pCAM1301-DOF-S + pROKII-JrDof3, pCAM1301-DOF-M1 + pROKII-JrDof3, pCAM1301-DOF-M2 + pROKII-JrDof3, represented the transformation of reporter DOFCOREZM motif, mutated DOFCOREZM motif, promoter segment including the DOFCOREZM motif, promoter segment containing the mutated DOFCOREZM motif, promoter segment excluding the DOFCOREZM motif transformed with the effecter, accordingly. Two asterisk means significant differences (p<0.01) between the negative control and others

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