Fig. 2

Time-related changes in the expression of HyPRP1 in response to V. dahliae (Vd) in G. hirsutum.a ND601 showed higher tolerence to V. dahliae (left panel) with lower disease index (right panel) compared to CCRI8 at 14 dpi. Two-week-old seedlings were dip-infected with the V. dahliae spores. The disease indices were presented means ± SE from three biological replications with at least 15 plants per replication. b, The expression of HyPRP1 changed to a lower degree both in stems and roots of tolerent ND601 and susceptible CCRI8 after infection using V. dahliae comparing to the control at the same hpi. Meanwhile, the relative expression of HyPRP1 displayed more lower both in stems and roots of tolerent ND601 comparing to the susceptible CCRI8 after infection using V. dahliae at the same hpi. The relative gene expression was calculated using the comparative 2^-ΔΔCt method with PP2A1 as endogenous control gene. Values are shown as the mean ± SE of three biological replicates. Sidak’s multiple comparisons test demonstrated that there were significant differences (**P < 0.01, ***P < 0.001) between Vd and control