Fig. 5

K-means clustering of differentially expressed transcripts along P. pinaster embryo development. Transcripts were clustered together according to their expression profiles and a representative mean expression profile (dashed line) was represented for every cluster. The 12 k-means clusters generated were further divided in four groups (A to D) depending on the embryo developmental stage in which the expression peaks. The inset displays the processes and functions found over-represented among the transcripts from the different clusters (and groups). a Clusters K1, K2, K3, K9, showing decreasing expression along development. Cellular carbohydrate metabolism, monosaccharide transport, carbohydrate metabolism, sterol metabolism, cell wall organization or biogenesis, external encapsulating structure organization,cell wall macromolecule metabolism, DNA-dependent DNA replication, biological regulation, organic cyclic compound metabolism, cellular aromatic compound metabolism, cellular component organization or biogenesis, cell cycle,developmental process, shoot system development, multicellular organismal process, mitotic cell cycle process, methylation, cell proliferation, microtubule-based process. b Clusters K6, K8, K10, K12, with expression peaking at Day11 and/or Day15. Steroid dehydrogenase activity, acting on the CH-CH group of donors, 3-oxo-5-alpha-steroid 4-dehydrogenase activity, abscisic acid catabolism, release of seed from dormancy. c Clusters K4, K5, with expression peaking at Day5. No GO-terms. d Clusters K7, K11, with increasing expression along development. Nutrient reservoir activity, carbon-oxygen lyase activity, acting on phosphates, carbon-oxygen lyase activity, alpha-bisabolene synthase activity, terpene synthase activity