Skip to main content
Fig. 4 | BMC Plant Biology

Fig. 4

From: Dosage imbalance of B- and C-class genes causes petaloid-stamen relating to F1 hybrid variation

Fig. 4

Comparative expression analyses of A-, B- and C-class genes. Real-time PCR were carried out in Petrocosmea glabristoma, P. sericea and their wild-type and petaloid-stamen mutant F1 hybrid flowers. SQU genes are expressed in all organs with an equal level in the mutant and wild-type as well as their parental flowers, despite the low expression level in the third floral whorl (a). LIP genes are universally expressed in all organs in the wild-type and mutant as well as their parental flowers (b). The transcripts of B-class genes DEF1 (c), DEF2 (d) and GLO (e) in both parental and F1 hybrid flowers are restricted in the second and third whorls, though slightly expansion into the fourth whorl in P. glabristoma flowers. However, the expression levels of both DEF2 and GLO genes are up-regulated in mutant flowers (d, e). The transcripts of PLE genes are restricted in the inner two whorls in both parental and F1 hybrid flowers with much lower expression level in the mutant than in the wild-type F1 hybrid flowers (f). ACTIN gene was amplified as an internal control. The values shown (mean ± SD) are the average of three biological replicates with each for three technical repeats. Asterisks indicate that mean values are significantly different between the mutants and their parents as well as their wild-type counterparts (P < 0.05). S, sepal; Dp, dorsal petal; Lp, lateral petal; Vp, ventral petal; St, stamen or petaloid-stamen; C, carpel; Pg, P. glabristoma; Ps, P. sericea; WT, wild-type; MU, mutant

Back to article page