Fig. 2

mRNA expression levels of barley HvIcy-2 gene in transgenic HvIcy2 silencing (KD Icy2, 1318, 1322, 1390 and 1399) lines, and wild type (WT) barley plants during darkness treatments (a) and M. oryzae infection (b), assayed by RT-qPCR. Total RNA was extracted from leaves after 14 days of darkness or 7 days of infection (dark grey) and non-treated/infected leaves (light grey). Data were expressed as mRNA levels of HvIcy2 gene normalized to barley cyclophilin mRNA content. Data are means ± standard error of at least three independent analyses. Different letters indicate significant differences between lines. (P < 0.01, one-way ANOVA followed by Student Newman-Keuls test)