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Fig. 2 | BMC Plant Biology

Fig. 2

From: Genome-wide identification, molecular evolution, and expression analysis of auxin response factor (ARF) gene family in Brachypodium distachyon L

Fig. 2

Subcellular localization of five BdARF proteins in Arabidopsis thaliana protoplasts. Five proteins included BdARF1, BdARF4, BdARF5, BdARF17 and BdARF19. The localization of the nuclei was detected by 4,6-diamidino-2-phenylindole (DAPI) staining. GFP: GFP fluorescence signal. Green fluorescence indicates the location of BdARFs in the Arabidopsis protoplasts; Chlorophyll: chlorophyll autofluorescence signal. Red fluorescent signal indicates the location of chloroplasts in protoplasts; DAPI: Blue fluorescence signal. Blue fluorescence indicates the location of the nucleus stained by DAPI; bright light: field of bright light; Merged: emergence of the GFP fluorescence signal, chlorophyll autofluorescence signal and bright light field; Nagtive: Wild-type (Clo) Arabidopsis protoplast cell. Scale bar = 5 μm

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