Fig. 9
From: Identification and characterization of GmMYB118 responses to drought and salt stress
GmMYB118 improves drought stress tolerance in transgenic soybean hairy roots. The seedlings with 2–5 cm hairy roots were grown for 5 days on pot under normal condition, then, the plants were dehydrated for 16 days. Survival rate of the water-stressed plants was monitored 3 days after rewatering. Image of drought resistance phenotypes of OE, CK and CRISPR plants under drought conditions was shown (a). The leaves contents of Chlorophyll (b), Proline (c) and MDA (d) were detected in OE, CK and CRISPR plants under drought or normal growth condition for a week. Trypan blue staining of soybean plant leaves without irrigation for 14 days (e), the dead cells can be strained, but living cells cannot. DAB (f) and NBT (g) staining of the leaves of OE, CK and CRISPR plants after drought treatment or normal condition for 7 days. The depth of color shows the concentration of H2O2 and O2− in the leaves (f-g). The content of H2O2 (h) and O2− (i) in the leaves of OE, CK and CRISPR plants after drought treatment or normal condition for 7 days. The data were means ± SDs of three experiments. ANOVA test demonstrated that there were significant differences (∗ P < 0.05, ∗∗ P < 0.01)