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Fig. 2 | BMC Plant Biology

Fig. 2

From: Comparative physiological responses and transcriptome analysis reveal the roles of melatonin and serotonin in regulating growth and metabolism in Arabidopsis

Fig. 2

a-g LBD16 and XTR6 are involved in melatonin- or serotonin-mediated LR development. a, b Five-day-old proLBD16:GUS (a) and proXTR6:GUS (b) seedlings were transferred to 1/4 MS medium containing 10 or 50 μM melatonin or serotonin for 4 days. c, d qRT-PCR analysis of LBD16 (c) and XTR6 (d) gene expression in the roots of Col-0 seedlings treated with 10 or 50 μM melatonin or serotonin for 2 days. The expression levels of the indicated genes in untreated roots were set to 1. e-h Effects of melatonin and serotonin on auxin accumulation in roots. e YFP fluorescence in the PR tips of 5-day-old DII-VENUS seedlings exposed to 1 μM IAA or 10 or 50 μM melatonin or serotonin for 4 days and (f) quantification of DII-VENUS fluorescence intensity in plants treated as in (e). g YFP fluorescence in the LR tips of 5-day-old DII-VENUS seedlings exposed to 1 μM IAA or 10 or 50 μM melatonin or serotonin for 4 days and (h) quantification of the DII-VENUS fluorescence intensity in plants treated as in (g). The fluorescence intensity in untreated roots was set to 1. MT, melatonin; ST, serotonin. Error bars represent the SE. Different letters indicate significantly different values (P < 0.05 by Tukey’s test)

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