Fig. 6

Molecular characterization of the rice T-DNA lines. a Expression levels of the gene of interest relative to its background line by qPCR: OsSCE1c.1 expression in 1A-23738 vs wild type Hwayoung (Hway), OsFUG1.1 expression in 2A-20225 vs Hwayoung; OsSAE1.1 expression in 3D-00611 vs wild type Dongjin; OsSCE1a.1 expression in 3A-05464 vs Dongjin; OsSIZ1.1–2 expression in 3A-02154 vs Dongjin; OsELS1.1 expression in 04Z11JY66 vs wild type Zhonghua11 (Zh11). Expression levels of each transcript in the T-DNA lines were normalized relative to its respective wild type (with a normalized value of 1). b-f T-DNA insertion localization site in the respective genes. White boxes – untranslated regions (UTR); black boxes – exons; lines – introns. b T-DNA in OsSCE1a knockdown (KD) line. c T-DNA in OsSCE1c knockout (KO) line. d T-DNA in OsELS1 KO line. e T-DNA in OsFUG1 KO line. f T-DNA in OsSIZ1 KD line is located in the 15th exon according to Wang et al. (2011). g-i Global levels of SUMOylation in the shoots of the T-DNA insertion lines in Western blots with anti-SUMO1, in normal growth conditions. T-DNA lines were compared to both wild type (WT) and negative segregant (NS) plants. Global SUMOylation levels in the KO lines of OsFUG1 and OsELS1 (g), in the T-DNA lines of OsSCE1c and OsSCE1a (h) and in OsSIZ1 KD line (i). HMWSC – High Molecular Weight SUMO Conjugates. LC – Loading Control (Coomassie blue staining). Free SUMO is marked with an asterisk