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Fig. 5 | BMC Plant Biology

Fig. 5

From: Insights into the transcriptional and post-transcriptional regulation of the rice SUMOylation machinery and into the role of two rice SUMO proteases

Fig. 5

The analysed SUMO proteases in rice: Os03g22440 (OsFUG1), Os01g25370 (OsELS1), Os05g11770 (OsSPF1), Os03g29630 (OsELS2) and Os01g53630 (OsOTS3). a Maximum likelihood phylogeny of the SUMO proteases family. Only nodes with bootstrap support > 75% show the correspondent bootstrap value. Os – Oryza sativa, Sc - Saccharomyces cerevisiae, At – Arabidopsis thaliana, Zm – Zea mays and Hv – Hordeum vulgare. Only the C48 peptidase domain was used to perform the alignments. b Venn diagram showing common CREs between five rice SUMO proteases: Os03g22440 (OsFUG1), Os01g25370 (OsELS1), Os05g11770 (OsSPF1), Os03g29630 (OsELS2) and Os01g53630 (OsOTS3). c Schematic representation of the SUMO proteases alternative splicing forms, showing three alternative splicing forms for OsFUG1 and OsELS1, and two for OsSPF1. White boxes – untranslated regions (UTR); black boxes – exons; lines – introns. Proteins lengths are indicated as well as the location of the catalytic triad of the C48 peptidase domain (histidine, aspartate and cysteine). d Basal expression levels of the different ASF of SUMO proteases genes performed by qPCR, in shoots (no pattern) and roots (patterned) in LC-93-4 (LC) and Nipponbare (Nipp)

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