Skip to main content
Fig. 3 | BMC Plant Biology

Fig. 3

From: Insights into the transcriptional and post-transcriptional regulation of the rice SUMOylation machinery and into the role of two rice SUMO proteases

Fig. 3

Analysis of the rice three genes of the E2 SUMO conjugating enzyme, OsSCE1a/b/c. a Maximum likelihood phylogeny of the SCE family. Only nodes with bootstrap support > 75% show the correspondent bootstrap value. Os – Oryza sativa, Sc - Saccharomyces cerevisiae, At – Arabidopsis thaliana, Zm – Zea mays and Hv – Hordeum vulgare. b Protein alignment of OsSCE1a, OsSCE1b and OsSCE1c. The catalytic cysteine in the active center is highlighted with “*”, and the E1 contact residues are highlighted with arrows. The residues that differ in Class II SCE from Class I are highlighted with “+”. c Venn diagram showing common CREs between OsSCEs. d Schematic representation of OsSCE alternative splicing forms, showing two for each OsSCE. White boxes – untranslated regions (UTR); black boxes – exons; lines – introns. The proteins lengths are indicated. High probability SUMOylation residues are indicated with an “S” in bold, whereas low probability SUMOylation residues are indicated with a normal “S”. e Basal expression levels of the different ASF of OsSCE1a/b/c genes by qPCR in shoots (no pattern) and roots (patterned) in LC-93-4 (LC) and Nipponbare (Nipp). OsSCE1b.1 and OsSCE1b.2 were quantified together since they could not be discriminated. f Transcript level profile of ASFs of OsSCE1a in response to 15 μM ABA

Back to article page