Fig. 3

Flowers of transgenic genome-edited torenia plants cultivated in a closed greenhouse and results of pigment analysis. a Four biallelic transgenic genome-edited torenia plants were grown in a closed greenhouse. Pictures of typical flowers are shown. CrV and CrW indicate cultivars Crown Violet and Crown White, respectively. CrV was the host plant for transformation, and CrW was a white cultivar for comparison. b Pigment analysis by spectrophotometry. Left panel, absorbance spectra of 0.1% HCl–methanol extracts of flower petals (line no. 5, CrV and CrW) are shown in the left panel. Right panel, relative anthocyanin contents of petals of transgenic genome-edited torenia plants and an untransformed control plant (CrV) were determined. Values indicate averages of five flower petals ± standard deviation