Table 1 Transgene copies of cry1Ac transgenic sugarcane by quantitative TaqMan real-time PCR

Group I

A1

26.25 ± 0.05

24.83 ± 0.77

24.10 ± 0.05^a

54.23 ± 1.7

0.46 ± 0.02

A2

26.21 ± 0.02

25.46 ± 0.20

24.10 ± 0.08^a

51.79 ± 0.55

0.49 ± 0.01

A3

26.13 ± 0.03

26.88 ± 0.64

24.37 ± 0.10^a

45.12 ± 3.13

0.60 ± 0.02

A4

26.24 ± 0.07

24.87 ± 1.20

24.37 ± 0.01^a

45.16 ± 0.43

0.55 ± 0.05

A5

26.04 ± 0.02

28.64 ± 0.40

24.28 ± 0.05a

48.02 ± 1.66

0.6 ± 0.01

A6

26.19 ± 0.07

25.75 ± 1.27

24.52 ± 0.05^a

40.62 ± 1.25

0.63 ± 0.05

B1

35.99 ± 0.32

0.03 ± 0.01

24.45 ± 0.05^a

42.66 ± 1.35

0.00 ± 0.00

B2

34.94 ± 0.11

0.04 ± 0.00

24.33 ± 0.04^a

46.35 ± 1.13

0.00 ± 0.00

B4

35.07 ± 0.11

0.04 ± 0.00

23.84 ± 0.08^a

65.22 ± 3.59

0.00 ± 0.00

I2

27.59 ± 0.06

8.05 ± 0.32

24.70 ± 0.06^a

35.92 ± 1.47

0.22 ± 0.02

I4

26.00 ± 0.03

24.63 ± 0.57

24.76 ± 0.08^a

34.48 ± 1.96

0.71 ± 0.03

Group II

D1

29.87 ± 0.04

1.59 ± 0.04

25.50 ± 0.01^a

20.7 ± 0.07

0.08 ± 0.00

D2

25.24 ± 0.06

35.60 ± 1.50

25.40 ± 0.03^a

22.14 ± 0.44

1.61 ± 0.12

D4

24.37 ± 0.01

63.47 ± 0.33

24.86 ± 0.01^a

32.01 ± 0.19

1.98 ± 0.02

K2

25.07 ± 0.01

39.78 ± 0.13

24.81 ± 0.02^a

33.26 ± 0.35

2.39 ± 0.01

K3

28.75 ± 0.027

3.37 ± 0.06

24.50 ± 0.03^a

41.2 ± 0.91

0.08 ± 0.00

K5

28.48 ± 0.02

4.04 ± 0.06

24.89 ± 0.07^a

31.45 ± 1.43

0.13 ± 0.00

Control

FN15

–

–

24.60 ± 0.05^a

38.54 ± 1.24

–

ROC22

–

–

24.51 ± 0.03^a

40.96 ± 0.87

–

ddH₂O

–

–

–

–

–