Fig. 7

Phenotypic analysis of GhbHLH282-overexpressing transgenic Arabidopsis plants in responses to 2, 4-epibrassinolide (BL). a-c Subcellular localization of GhbHLH282 protein. a Fluorescence of eGFP:GhbHLH282 fusion proteins in the tobacco (Nicotiana Benthamiana) foliar epidermis cells. b Nuclear DAPI staining of the same cell in a. c The images of a and b were merged over the bright-field. d Semiquantitative RT-PCR analysis of GhbHLH282 expression in the transgenic plants and wild type controls. Transcript levels of GhbHLH282 were determined by Semiquantitative RT-PCR analysis, using AtACTIN2 as a quantification control. e The phenotypes of GhbHLH282-overexpressing seedlings and wild type grown on half-strength MS medium without BL for 7 days. f The phenotypes of GhbHLH282-overexpressing seedlings and wild type grown on half-strength MS medium with 1000 nM BL for 7 days. g Statistical analysis of petiole length of the GhbHLH282-overexpressing transgenic lines and wild type. The petiole growth of GhbHLH282-overexpressing plants showed the decreased sensitivity to BL, compared with mock control (0 nM BL), when treated with 10, 100 and 1000 nM BL. h Statistical analysis of root length of the GhbHLH282-overexpressing transgenic lines and wild type. The root growth of GhbHLH282-overexpressing plants showed the increased sensitivity to BL, compared with mock control (0 nM BL), when treated with 10, 100 and 1000 nM BL. Mean values and standard deviations are shown from three independent experiments. One or two asterisks represent there was significant (P < 0.05) or very significant (P < 0.01) difference in petiole length or root length between the BL-treated sample and the untreated sample (control), respectively. WT, wild type; L1, L4 and L6, three GhbHLH282 transgenic lines