“Expression of genes encoding protein disulfide isomerase (PDI) in cultivars and lines of common wheat with different baking quality of flour”

Table 4 Characterization of outer primers used for nested PCR in wheat

Gene	Name	Sequence 5′-3′	Product size	Annealing temp.
PDI	QFnest	GAGTATGAGAAGGCGGCCC	983 bp	51 °C
PDI	QRnest	AGAACATTTTTGCCAGATTTGA	983 bp	51 °C
actin	AFnest	ACAACTGGGATGACATGGGGAA	435 bp	53 °C
actin	ARnest	CAAGGGCCACGTAAGCGA	435 bp	53 °C
CDC48	CFnest	TTAGGCCAGGGCGTCTTGA	542 bp	54 °C
CDC48	CRnest	AGTGAAGGGCACAACAACGAG	542 bp	54 °C

ISSN: 1471-2229