Fig. 5

The thermal stability of the CdDHN4 protein. CD spectra of CdDHN4-L (a) and CdDHN4-S (b) to analyze the effect of increasing temperature on the protein secondary structures. The spectra were measured in 10°Cincrements in deionized water, pH 7.0, from 10 to 90 °C. c Difference spectra obtained after subtracting the spectra of both proteins from spectra obtained at 10 and 90 °C. d The changes of random ratio in CdDHN4-L or S with increasing temperature. e CdDHN4-L and CdDHN4-S and BSA were (+) or were not (−) incubated at 100 °C for 10 min. The samples were sediment with centrifugation 12000 rpm 30 min, and the supernatants were run on SDS-PAGE