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Fig. 3 | BMC Plant Biology

Fig. 3

From: The type 2 acyl-CoA:diacylglycerol acyltransferase family of the oleaginous microalga Lobosphaera incisa

Fig. 3

Restoring of TAG synthesis in H1246 yeast mutant by L. incisa DGATs heterologous expression. a LDs formation analyzed by Bodipy staining (green) in H1246 cells transformed with empty vector and H1246 cells expressing single (LiDGAT1, LiDGAT2.1, LiDGAT2.2 and LiDGAT2.3) and double (LiDGAT1xLiDGAT2.2, LiDGAT2.1xLiDGAT2.2 and LiDGAT2.2xLiDGAT2.3) LiDGAT-encoding constructs. Bars 5 μm. b Quantification of TAG levels extracted from transformed yeast showed on (a). c Changes in the TAG fatty acid profile in response to expression of empy vector (EV) and constructs from (a). For experiments showed in (b) and (c) values represent mean ± SD (n = 3). Raw data for TAG content and FA composition are given in Additional file 11

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