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Fig. 3 | BMC Plant Biology

Fig. 3

From: Integrated physiologic, genomic and transcriptomic strategies involving the adaptation of allotetraploid rapeseed to nitrogen limitation

Fig. 3

Transcriptional profiling of the phenylpropanoid pathway for the anthocyanin biosynthesis and rapeseed leaves/stems accumulating anthocyanin. a, b Transcriptional profiling of the chlorophyll-binding protein genes (a) and the phenylpropanoid pathway for the anthocyanin biosynthesis (b) in the shoots under sufficient N supply (0 h) and long-term N limitation (72 h) conditions. Enzymes in each step: PAL, phenylalanine ammonia lyase; C4H, cinnamic acid 4-hydroxylase; 4CL, coumaroyl-CoA synthase; CHS, chalcone synthase; CHI, chalcone-flavanone isomerase; F3H, flavanone 3-hydroxylase; F3’H, flavanone 3′-hydroxylase; DRF, dihydroflavonol 4-reductase; ANS, anthocyanidin synthase; AGT, anthocyanin glycosyltransferase. Each column indicates a gene. c, d Number of the differentially expressed genes (DEGs) involved in the anthocyanin biosynthesis (c) and MYB transcription factors (d). Up, up-regulation; down: down-regulation; n.s., not significant. e Transcriptional profiling of the Production of Anthocyanin Pigment (PAP) genes BnaPAP1s (BnaMYB75s) and BnaA7.PAP2 (BnaA7.MYB90) under long-term N limitations. f Relative expression of BnaA7.PAP2 under N limitations by the qRT-PCR assay. Heat maps of gene expression profiling were generated using Multi-experiment Viewer (Mev, http://www.mybiosoftware.com/mev-4-6-2-multiple-experiment-viewer.html). False discovery rate (FDR) R) ultiown-log2(fold-change) ≥ 1 were used as the thresholds to identify DEGs. Regarding the RNA-seq experiment and qRT-PCR assays, the rapeseed plants that were grown under 9.0 mM NO3 for 10 d were then transferred to 0.30 mM NO3, and the shoots and roots were individually sampled at 0 h, 3 h and 72 h, respectively. The color scales of heat maps indicate the expression levels (FPKM values) or fold-changes of gene expression, and the differentially expressed genes between the control (0 h) and the long-term N limitation treatment (72 h) are indicated by asterisks. (g, h) Rapeseed leaves (g) and stems (h) with accumulated anthocyanin. The rapeseed plants that were hydroponically cultivated under high (9.0 mM) NO3 condition for 10 d were then transferred to 9.0 mM and 0.30 mM NO3 conditions for 10 d

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