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Fig. 2 | BMC Plant Biology

Fig. 2

From: Integrated physiologic, genomic and transcriptomic strategies involving the adaptation of allotetraploid rapeseed to nitrogen limitation

Fig. 2

Genome-wide identification and characterization of the differentially expressed genes (DEGs) that were responsive to nitrogen (N) limitations. a Pearson correlation coefficients of the RNA-seq data between each pair of biological replicates. S and R indicate shoots and roots; S0/R0, S3/R3 and S72/R72 indicate shoots/roots at 0 h, 3 h and 72 h, respectively. b-c Venn diagram showing intersection analysis (b) and gene ontology (GO) term annotations of the DEGs. In the word cloud, the font sizes indicate the GO term numbers. The bigger the fonts are, the more the corresponding GO terms are. d-g KEGG enrichment analysis of the DEGs in the shoots (d, e) and roots (f, g) at 3 h and 72 h. The solid circle sizes represent the pathway enriched degree. The bigger the circles are, the more the corresponding KEGG items are. Regarding the RNA-seq experiment, the rapeseed plants that were grown under 9.0 mM NO3 for 10 d were then transferred to 0.30 mM NO3, and the shoots and roots were individually sampled at 0 h, 3 h and 72 h. False discovery rate (FDR) ≤ 0.05 and log2 (fold-change) ≥ 1 are used as the thresholds to identify DEGs

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