Table 3 Primers used in the experiments
Gene | Direction | Sequence (5′-3′) | Function |
|---|---|---|---|
CsMYB2 | Forward | ATGGGGAGAAGCCCTTGTTGCGCG | Full-length clone |
Reverse | CTAGACAAATTGCCAATCCCCTCC | ||
CsMYB26 | Forward | ATGGGGAGGAGTCCATGCTGC | Full-length clone |
Reverse | TCATGGCCAGTCCTCAGAATCAAG | ||
CsMYB2 | Forward | TTACAGCAATGACAACAA | RT-qPCR |
Reverse | CCAGACTCCAGAATAGAA | ||
CsMYB26 | Forward | CTCCAACTCCATAATATCAA | RT-qPCR |
Reverse | GAACAAGGTATCGTCATAA | ||
CsDFR | Forward | TGCAGAGAGAAGGGTTTGCT | RT-qPCR |
Reverse | AAGGCAAGGCACCAATACAC | ||
CsANS | Forward | TCGAGCCCTAGCTACCAAGA | RT-qPCR |
Reverse | CAAGTCAGTGTGGGCTTCAA | ||
CsANR | Forward | GCCTGGTCATGGATGAGAGT | RT-qPCR |
Reverse | GGCCATGAGAGTAGGGATGA | ||
CsLAR | Forward | GGGGCATCCTGTATCAAAGA | RT-qPCR |
Reverse | CCGCATACCTTTCAGTCCAT | ||
CsUFGT | Forward | GCACCATAACCACCCCACC | RT-qPCR |
Reverse | TGTCACAAACACACCAACCGAT | ||
CsF3’H | Forward | CTATTGCAGCTTCTTGATGATCCGA | RT-qPCR |
Reverse | GCTCTTTGGTTGCTTTGTTGATTAG | ||
CsMYB2 | Forward | CACCATCACCATCACGCCATGATGGGGAGAAGCCCTTGTTGCGCG | Subcellular localization |
Reverse | CACTAGTACGTCGACCATGGCGACAAATTGCCAATCCCCTCC | ||
GAPDH | Forward | TTGGCATCGTTGAGGGTCT | Reference gene |
Reverse | CAGTGGGAACACGGAAAGC |