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Fig. 4 | BMC Plant Biology

Fig. 4

From: Wheat Bax Inhibitor-1 interacts with TaFKBP62 and mediates response to heat stress

Fig. 4

The co-localization between TaBI-1.1 and TaFKBP62 and TaFKBP62 expression patterns under stress treatments. a The subcellular localization of TaFKBP62 and the co-localization between TaBI-1.1 and TaFKBP62 in wheat protoplasts. The left panel shows the fluorescence of GFP, mRFP and the merge. Bars = 10 μm. The right panel shows the co-localization levels calculated from the overlap coefficients obtained from at least ten individual protoplasts. b TaFKBP62 expression patterns after heat treatment for 0, 0.5, 1, 2, 4, 8, 12, and 24 h. Wheat Actin was used as a reference. The vertical coordinates represent the fold-changes, and the horizontal ordinates represent the different time periods. The results are shown as the means±SD of three biological replicates. Error bars indicate the SD. c The phenotypes of Col-0, 35S::TaFKBP62–1, 35S::TaFKBP62–2, and 35S::TaFKBP62–3 after heat stress. d The survival rates of Col-0, 35S::TaFKBP62–1, 35S::TaFKBP62–2, and 35S::TaFKBP62–3 under heat stress. The results are shown as the means±SD of three biological replicates. Error bars indicate the SD. e The relative conductivity of Col-0, 35S::TaFKBP62–1, 35S::TaFKBP62–2, and 35S::TaFKBP62–3 after heat treatment. The results are shown as the means±SD of six biological replicates. Error bars indicate the SD

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