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Fig. 7 | BMC Plant Biology

Fig. 7

From: Pervasive read-through transcription of T-DNAs is frequent in tobacco BY-2 cells and can effectively induce silencing

Fig. 7

Transcription of GFP gene in siliques of selected Arabidopsis thaliana transformants with pER8-GFP. RT-qPCR analysis of transcript levels in five selected plants untreated with β-estradiol. a The level of the sense GFP transcript (RT: R1 primer, qPCR: F1 and R1 primers); b the level of the sense transcript containing the region 50 nt upstream of transcription start site (TSS) of the inducible promoter (RT: R1 primer, qPCR: F2 and R2 primers); c the level of the antisense GFP transcript (RT: F1 primer, qPCR: F1 and R1 primers); d the level of the antisense transcript containing the region 50 nt downstream of the last poly(A) signal of T3A terminator (RT: F1 primer, qPCR: F3 and R3 primers)

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