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Fig. 2 | BMC Plant Biology

Fig. 2

From: Pervasive read-through transcription of T-DNAs is frequent in tobacco BY-2 cells and can effectively induce silencing

Fig. 2

Frequency of spontaneous silencing of GFP in supertransformed BY-2 calli. Silencer and control T-DNAs (as described in Fig. 1) were supertransformed to BY-2 cell line stably expressing GFP. The fluorescence was assessed in independently transformed calli grown on non-inductive medium six weeks after transformation. The bars represent the percentage of fluorescent non-silenced calli (the lower part of the bar) and silenced calli (the upper part of the bar). The variants AS-GFP and UT-GFP represent averages of three biological replicas and the variants GFP and IR-GFP represent averages of four biological replicas (independent transformations). Each replica had 200 calli (with exception of EV with 160 calli). Variants that significantly differed from the EV control are marked with ** (p < 10− 6). ΔP1: pER8-IR-GFPΔP1; ΔP2: pGreen-IR-GFPΔP2; EV: empty vector pER8

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