Fig. 1
Schematic representation of T-DNAs used in this study. Functional elements, positions of primers used for reverse transcription and qPCR, position of a probe and restriction enzyme sites used for Southern hybridization are shown above and below the appropriate sequences. pCP60-GFP: construct for expression of GFP under the constitutive promoter. pER8: a set of T-DNAs used for the inducible expression: pER8-AS-GFP: sequence of GFP in antisense orientation; pER8-UT-GFP: sequence of GFP without terminator; pER8-IR-GFP: sequence of GFP arranged as inverted repeat; pER8-IR-PIN3: sequence of PIN3 gene arranged as inverted repeat; pER8-GFP: sense GFP. The pER8-IR-GFPΔP1 and pGreen-IR-GFPΔP2 are the two promoterless controls with GFP sequence arranged as inverted repeat. RB: right border, LB: left border, PNOS: nopaline synthase promoter, P35S: 35S promoter, PG10–90: synthetic constitutive promoter; PIND: inducible promoter activated by β-estradiol; TNOS: nopaline synthase terminator; TE9: rbcS E9 terminator; T3A: rbcs S 3A terminator; XVE: estrogen receptor and transcriptional activator; NPTII: kanamycin resistance gene; HPT: hygromycin resistance gene; GFP: green fluorescent protein coding sequence; intron: intron from Solanum tuberosum PsbO gene; LacZ: fragment of bacterial β–galactosidase gene. All T-DNAs are at the same scale as indicated with 1kbp scale bar