TY - JOUR AU - Ebersbach, Paul AU - Stehle, Felix AU - Kayser, Oliver AU - Freier, Erik PY - 2018 DA - 2018/11/12 TI - Chemical fingerprinting of single glandular trichomes of Cannabis sativa by Coherent anti-Stokes Raman scattering (CARS) microscopy JO - BMC Plant Biology SP - 275 VL - 18 IS - 1 AB - Cannabis possesses a rich spectrum of phytochemicals i.e. cannabinoids, terpenes and phenolic compounds of industrial and medicinal interests. Most of these high-value plant products are synthesised in the disk cells and stored in the secretory cavity in glandular trichomes. Conventional trichome analysis was so far based on optical microscopy, electron microscopy or extraction based methods that are either limited to spatial or chemical information. Here we combine both information to obtain the spatial distribution of distinct secondary metabolites on a single-trichome level by applying Coherent anti-Stokes Raman scattering (CARS), a microspectroscopic technique, to trichomes derived from sepals of a drug- and a fibre-type. SN - 1471-2229 UR - https://doi.org/10.1186/s12870-018-1481-4 DO - 10.1186/s12870-018-1481-4 ID - Ebersbach2018 ER -