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Fig. 7 | BMC Plant Biology

Fig. 7

From: OsWRKY67 positively regulates blast and bacteria blight resistance by direct activation of PR genes in rice

Fig. 7

OsWRKY67 binds specifically to the W-box elements. a Yeast one-hybrid assays indicated that OsWRKY67 can bind directly to the sequences of W-box (BS65) and its own promoter (67p). P53 is the positive control. NC is the negative control which did not contain the W-box and mBS65 is the mutated probe of BS65 with TGAC sequence changed to CCTA. The detailed sequences of BS65 and mBS65 are shown in section c. b Yeast one-hybrid assays indicated that OsWRKY67 can bind directly to the promoters of PR1a and PR10 through the W-box motif. NC is the negative control from the PR10 promoter which did not contain the W-box motif. c The EMSA experiment showed the binding of OsWRKY67 protein to the sequences of the W-box. An excess of unlabeled-BS65 probe was added as competitors. The negative control was conducted by inoculating the Biotin-labeled BS65 probe without the OsWRKY67-His protein. d The EMSA experiment showed the binding of OsWRKY67 protein to its own promoter through the W-box element. The oligonucleotides 67p and m67p were used as the probes. An excess of unlabeled-67p probe was added as competitors. e The EMSA experiment showed the binding of OsWRKY67 protein to the promoter of PR10. The oligonucleotides P10 and mP10 were used as the probes. An excess of unlabeled-P10 probe was added as competitors. f The EMSA experiment showed the binding of OsWRKY67 protein to the promoter of PR1a. The oligonucleotides P1 and mP1 were used as the probes. An excess of unlabeled-P1 probe was added as competitors

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