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Table 1 Activity of PtNIT1 with different aromatic and aliphatic nitriles as substrates. The purified recombinant protein was incubated with the potential nitrile substrate and the formation of the corresponding acids was analyzed using LC-MS/MS

From: The nitrilase PtNIT1 catabolizes herbivore-induced nitriles in Populus trichocarpa

substrate substrate structure activity
β-cyanoalanine yes
3-phenylpropionitrile yes
benzyl cyanide yes
4-hydroxybenzyl cyanide yes
indole-3-acetonitrile yes
isovaleronitrile no
3-butenenitrile no
butyronitrile no