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Table 1 Activity of PtNIT1 with different aromatic and aliphatic nitriles as substrates. The purified recombinant protein was incubated with the potential nitrile substrate and the formation of the corresponding acids was analyzed using LC-MS/MS

From: The nitrilase PtNIT1 catabolizes herbivore-induced nitriles in Populus trichocarpa

substrate

substrate structure

activity

β-cyanoalanine

yes

3-phenylpropionitrile

yes

benzyl cyanide

yes

4-hydroxybenzyl cyanide

yes

indole-3-acetonitrile

yes

isovaleronitrile

no

3-butenenitrile

no

butyronitrile

no