Fig. 5
From: Modulation of auxin and cytokinin responses by early steps of the phenylpropanoid pathway
t-CA promotes leaf expansion and petiole elongation. a-d Kinematic growth analysis of the third (L3) and the fifth (L5) leaf of Col-0 plants grown on control media or media with 1 μM t-CA. Error bars represent mean ± SD (n ≥ 8). The significant differences between t-CA-treated and control samples for each day is noted (**, P < 0.01; ****, P < 0.0001; two-way ANOVA with Bonferroni’s multiple comparisons test). e Piperonylic acid (PA) treatments differentially affect growth of the wild-type and KMD1/KFB20 overexpression (OE#1) plants. Plants were grown on MS/2 media supplemented with the denoted doses of PA for 18 days. Due to the size difference of the rosettes of the tested lines, the fresh weight (FW) was presented as normalized mean ± SD (n ≥ 6, each sample containing 6 seedlings) with the mean FW for each line grown on control media being assigned the value of 1. The significance of the difference between the control and treatments is noted for each line (ns, not significant, ****, P < 0.0001; two-way ANOVA with Bonferroni’s multiple comparisons test). f Size increase in kfb20–1 kfb1–1 kfb50–1 (kfb tr.) plants treated with the denoted doses of t-CA. Experimental setup and data analyses are as described in e. The significance of the difference between treated and untreated plants for each line is shown (****, P < 0.0001; two-way ANOVA with Bonferroni’s multiple comparisons test)