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Fig. 6 | BMC Plant Biology

Fig. 6

From: Overexpression of PvCO1, a bamboo CONSTANS-LIKE gene, delays flowering by reducing expression of the FT gene in transgenic Arabidopsis

Fig. 6

The PvCO1-PvGF14c interaction by yeast two-hybrid (a) and pull down (b) assays. a The pGBKT7–53 and pGADT7-T interaction was used as a positive control. The pGBKT7-lam and pGADT7-T interaction was used as a negative control. The PvCO1 and empty interaction was used as self-activation test. PvCO1 indicated full length ORF of PvCO1 gene. PvCO1(B-box) indicated the truncated CO protein containing the two B-box domain. PvCO1(CCT) indicated the truncated CO protein containing the CCT domain. b The addition of GST or GST-PvGF14c is indicated in each line. The antibodies used for Western blotting are abbreviated as WB:α-His and WB:α- GST. Input, the proteins from whole cell extracts; Pull-down, the pull down proteins

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BMC Plant Biology

ISSN: 1471-2229

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