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Fig. 1 | BMC Plant Biology

Fig. 1

From: OsPHR3 affects the traits governing nitrogen homeostasis in rice

Fig. 1

Tissue-specific differential relative expression levels of OsPHR3 during growth under different regimes of N variants. Seeds of the wild-type were grown hydroponically in IRRI solution for 2 weeks, starved for N for 3 d and then supplied for 24 h with nutrient solution containing high NH4+ (H NH4+, 5 mM), low NH4+ (L NH4+, 0.25 mM), high NO3 (H NO3, 5 mM), low NO3 (L NO3, 0.25 mM), 2.5 mM N (1.25 mM NH4+ and 1.25 mM NO3,) and 0.25 mM (0.125 mM NH4+ and 0.125 mM NO3). Root and shoot were harvested for the qRT-PCR analysis of the relative expression levels of OsPHR3 in (a) high and low NH4+ or NO3 and (b) 2.5 mM N and 0.25 mM N conditions. Actin (OsRac1; LOC_Os03g50885) was used as an internal control and the values for H NH4+, H NO3 and + N were normalized to 1. Values are means ±SE (n = 3) and different letters on the histograms indicate that the values differ significantly (P < 0.05, one-way ANOVA)

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