Fig. 5
Expression of representative DEGs in multiple pathways by RT-qPCR. The Sample H7 was as a reference sample that was from the seventh day after the start point of oil accumulation in Tree H. a Glycolysis; b Fatty acid biosynthesis; c TAG assembly; d Fatty acid degradation; e Pyruvate metabolism. L indicated Tree L, with low final oil seed content; H indicated Tree H, with high final seed oil content. Times in legend indicated time after start point of oil accumulation. Abbreviations: FPKM, reads per kilobase of exon model per million mapped reads (FPKM); ENO2, enolase; i-PGM, 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, PEG-2, putative glucose-6-phosphate 1-epimerase; PK2, plastidial pyruvate kinase 2; KAR, ketoacyl-ACP reductase; ENR, enoyl-ACP reductase; KAS I, ketoacyl-ACP synthase I, LASC9, long chain acyl-CoA synthetase 9; GPDH, glycerol-3-phosphate dehydrogenase; DGAT2, type 2 diacylglycerol acyltransferase; LPAA2, 1-acyl-sn-glycerol-3-phosphate acyltransferase 2: FADX, fatty acid conjugase/desaturase; MFP2, MULTIFUNCTIONAL family protein; ACX4, Acyl-coenzyme A oxidase 4;, KAT2, 3-ketoacyl-CoA thiolase 2; LACS7, Long chain acyl-CoA synthetase 7; ADH, alcohol dehydrogenase 1; ALDH2, aldehyde dehydrogenase family 2, ALDH7, aldehyde dehydrogenase family 7; PCK, phosphoenolpyruvate carboxykinase