Fig. 6

Levels of histone H3ac, H3K4me2, and H3K4me3 under cold and heat treatments. a Western blot showing the H3ac, H3K4me2, and H3K4me3 status in soybean leaves treated with cold and heat. 10-day-old seedlings were treated under cold (4 °C) and heat (42 °C) conditions for 12 h and the leaves were sampled for total protein extraction. b Quantification of western blot results. Signal intensities were measured using the ImageJ software and normalized to the loaded amount of H3. Values are expressed as fold change over control treatment. Shown is the mean ± SEM of three independent experiments. *, P-value < 0.05, Student’s t-test