Fig. 5
SNFL1 expression pattern and protein subcellular localization. (a) RT-PCR analysis of SNFL1 transcripts in different rice tissues. Roots (R) and culms (c) were harvested from 2-month-old WT plants. Flag leaf (FL), leaf sheaths (LS) and young panicles (YP) were collected at the booting stage. (b–h) GUS staining of PROSNFL1:GUS transgenic line tissues. (Scale bar, 1 cm.) (b) Root. (c) Culm. (d) Leaf blade. (e) Leaf sheath. (f–h) Young panicles of different lengths. (i) Subcellular localization of SNFL1-GFP fusion protein in rice protoplasts. (Scale bar, 5 μm)