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Table 5 Identification of the up-regulated unigenes annotated and related to metabolic resistance in Myosoton aquaticum by RNA-Seq and qRT-PCR (2-ΔCt)

From: Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.)

Gene ID

PFAM ID

Function annotation

RNA-Seq

FoldChage: qRT-PCR (2-ΔCt)

Padja

FoldChange (MRT_ST)

P-valueb

RNA-Seq samples (MRT_ST)c

c47752_g3

PF00067.17

CytP450, CYP716B1

2.40E-02

2.41

1.81E-01

1.24(0.17)

c49980_g3

PF00067.17

CytP450, CYP71A21

2.37E-02

3.24

6.00E-03

0.56(0.14)

c31888_g1

PF00067.17

CytP450, CYP72A219

7.73E-05

2.16

1.00E-03

0.45(0.08)

c28525_g1

PF00067.17

CytP450, CYP734A1

6.32E-07

2.28

5.00E-03

1.94(0.2) **

c49866_g6

.

CytP450, CYP76C1

3.73E-02

2.19

3.67E-04

3.22(0.34)***

c44104_g1

PF00067.17

CytP450, CYP82A4

2.69E-02

3.4

7.10E-02

0.91(0.06)

c48448_g1

PF00067.17

CytP450, CYP86B1

4.16E-06

2.04

1.00E-03

1.27(0.03)**

c14606_g1

PF00067.17

CytP450, CYP94A1

2.67E-01

1.49

1.31E-01

1.46(0.25)

c50084_g2

PF00032.12

Cytochrome b6-f

1.68E-02

5.22

4.30E-02

0.85(0.08)

c49404_g2

PF01578.15

Cytochrome c

2.56E-02

5.17

9.04E-01

1.02(0.17)

c48737_g9

.

Cytochrome c1–2

1.72E-02

2.47

4.30E-02

0.54(0.12)

c27216_g1

PF13417.1

GST, T1

8.89E-01

1.09

4.40E-01

0.89(0.18)

c33248_g1

PF03552.9

Glycosyltransferase, GT28

1.94E-04

2.79

1.00E-03

0.13(0.01)

c33752_g1

PF00295.12

Glycosyltransferase, GT28

3.39E-02

3.26

3.00E-03

0.27(0.06)

c47986_g3

PF00201.13

UDP-glycosyltransferase, UGT73B3

4.04E-07

3.48

3.16E-01

0.89(0.13)

c32196_g1

PF00201.13

UDP-glycosyltransferase, UGT78D2

8.88E-01

1.07

1.30E-01

1.28(0.25)

c40202_g1

PF04577.9

Glucosamine transferase

6.23E-03

2.37

6.24E-01

0.95(0.16)

c30791_g1

.

ABC transporter, ABCB2

1.34E-04

2.15

2.00E-03

0.65(0.05)

c49741_g6

.

ABC transporter, ABCB2

6.75E-13

2.62

1.00E-03

2.29(0.2) **

c49337_g1

PF03468.9

ABC transporter, ABCB29

6.96E-04

2.8

9.20E-02

0.7(0.06)

c45895_g2

.

ABC transporter, ABCC10

1.05E-03

2.45

2.00E-03

1.96(0.2)**

c45895_g3

.

ABC transporter, ABCC10

5.12E-07

4.89

9.20E-02

0.76(0.04)

c50054_g1

PF00005.22

ABC transporter, ABCC10

9.18E-06

2.61

2.10E-02

2.16(0.53) *

c39205_g1

PF00664.18

ABC transporter, ABCC3

4.55E-11

2.16

7.50E-02

1.54(0.33) **

c47115_g3

PF00664.18

ABC transporter, ABCC8

2.97E-02

2.67

6.30E-02

0.67(0.04)

c37150_g1

PF00403.21

Peroxidase

5.01E-06

2.51

7.00E-03

0.59(0.13)

c44363_g1

PF00141.18

Peroxidase 5

7.23E-25

6.74

1.56E-04

0.38(0.07)

c33094_g1

PF00141.18

Peroxidase 57

3.49E-14

3.94

1.30E-02

1.93(0.34) *

c42442_g1

PF00141.18

Peroxidase 57

1.15E-06

1.79

2.39E-04

1.73(0.07) ***

c44336_g7

.

Oxidase

2.75E-02

4.2

1.00E-03

0.54(0.02)

c40277_g1

PF00657.17

Esterase

6.59E-23

3.88

2.00E-03

0.44(0.05)

c48393_g3

.

Esterase

2.56E-04

3.7

3.81E-01

0.85(0.22)

c35829_g1

PF00722.16

Hydrolase

1.24E-04

4.59

8.90E-02

0.82(0.11)

c50023_g1

PF10551.4

Hydrolase

2.51E-08

4.73

6.00E-02

1.12(0.05)

  1. MR_T, resistant M. aquaticum L seedlings sprayed tribenuron-methyl; S_T, susceptible M. aquaticum L seedlings sprayed tribenuron-methyl
  2. aThe resulting p-value was adjusted and expressed as the padj by the Benjamini-Hochberg procedure for controlling the false discovery rate
  3. bMeans were separated using Fisher’s protected least significant difference (LSD) test at the 5% level of probability (from SPSS analysis)
  4. cP-value of < 0.05, 0.01, 0.001 is indicated by *, **, and ***, respectively (from SPSS analysis)