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Table 5 Identification of the up-regulated unigenes annotated and related to metabolic resistance in Myosoton aquaticum by RNA-Seq and qRT-PCR (2-ΔCt)

From: Non-target site-based resistance to tribenuron-methyl and essential involved genes in Myosoton aquaticum (L.)

Gene ID PFAM ID Function annotation RNA-Seq FoldChage: qRT-PCR (2-ΔCt)
Padja FoldChange (MRT_ST) P-valueb RNA-Seq samples (MRT_ST)c
c47752_g3 PF00067.17 CytP450, CYP716B1 2.40E-02 2.41 1.81E-01 1.24(0.17)
c49980_g3 PF00067.17 CytP450, CYP71A21 2.37E-02 3.24 6.00E-03 0.56(0.14)
c31888_g1 PF00067.17 CytP450, CYP72A219 7.73E-05 2.16 1.00E-03 0.45(0.08)
c28525_g1 PF00067.17 CytP450, CYP734A1 6.32E-07 2.28 5.00E-03 1.94(0.2) **
c49866_g6 . CytP450, CYP76C1 3.73E-02 2.19 3.67E-04 3.22(0.34)***
c44104_g1 PF00067.17 CytP450, CYP82A4 2.69E-02 3.4 7.10E-02 0.91(0.06)
c48448_g1 PF00067.17 CytP450, CYP86B1 4.16E-06 2.04 1.00E-03 1.27(0.03)**
c14606_g1 PF00067.17 CytP450, CYP94A1 2.67E-01 1.49 1.31E-01 1.46(0.25)
c50084_g2 PF00032.12 Cytochrome b6-f 1.68E-02 5.22 4.30E-02 0.85(0.08)
c49404_g2 PF01578.15 Cytochrome c 2.56E-02 5.17 9.04E-01 1.02(0.17)
c48737_g9 . Cytochrome c1–2 1.72E-02 2.47 4.30E-02 0.54(0.12)
c27216_g1 PF13417.1 GST, T1 8.89E-01 1.09 4.40E-01 0.89(0.18)
c33248_g1 PF03552.9 Glycosyltransferase, GT28 1.94E-04 2.79 1.00E-03 0.13(0.01)
c33752_g1 PF00295.12 Glycosyltransferase, GT28 3.39E-02 3.26 3.00E-03 0.27(0.06)
c47986_g3 PF00201.13 UDP-glycosyltransferase, UGT73B3 4.04E-07 3.48 3.16E-01 0.89(0.13)
c32196_g1 PF00201.13 UDP-glycosyltransferase, UGT78D2 8.88E-01 1.07 1.30E-01 1.28(0.25)
c40202_g1 PF04577.9 Glucosamine transferase 6.23E-03 2.37 6.24E-01 0.95(0.16)
c30791_g1 . ABC transporter, ABCB2 1.34E-04 2.15 2.00E-03 0.65(0.05)
c49741_g6 . ABC transporter, ABCB2 6.75E-13 2.62 1.00E-03 2.29(0.2) **
c49337_g1 PF03468.9 ABC transporter, ABCB29 6.96E-04 2.8 9.20E-02 0.7(0.06)
c45895_g2 . ABC transporter, ABCC10 1.05E-03 2.45 2.00E-03 1.96(0.2)**
c45895_g3 . ABC transporter, ABCC10 5.12E-07 4.89 9.20E-02 0.76(0.04)
c50054_g1 PF00005.22 ABC transporter, ABCC10 9.18E-06 2.61 2.10E-02 2.16(0.53) *
c39205_g1 PF00664.18 ABC transporter, ABCC3 4.55E-11 2.16 7.50E-02 1.54(0.33) **
c47115_g3 PF00664.18 ABC transporter, ABCC8 2.97E-02 2.67 6.30E-02 0.67(0.04)
c37150_g1 PF00403.21 Peroxidase 5.01E-06 2.51 7.00E-03 0.59(0.13)
c44363_g1 PF00141.18 Peroxidase 5 7.23E-25 6.74 1.56E-04 0.38(0.07)
c33094_g1 PF00141.18 Peroxidase 57 3.49E-14 3.94 1.30E-02 1.93(0.34) *
c42442_g1 PF00141.18 Peroxidase 57 1.15E-06 1.79 2.39E-04 1.73(0.07) ***
c44336_g7 . Oxidase 2.75E-02 4.2 1.00E-03 0.54(0.02)
c40277_g1 PF00657.17 Esterase 6.59E-23 3.88 2.00E-03 0.44(0.05)
c48393_g3 . Esterase 2.56E-04 3.7 3.81E-01 0.85(0.22)
c35829_g1 PF00722.16 Hydrolase 1.24E-04 4.59 8.90E-02 0.82(0.11)
c50023_g1 PF10551.4 Hydrolase 2.51E-08 4.73 6.00E-02 1.12(0.05)
  1. MR_T, resistant M. aquaticum L seedlings sprayed tribenuron-methyl; S_T, susceptible M. aquaticum L seedlings sprayed tribenuron-methyl
  2. aThe resulting p-value was adjusted and expressed as the padj by the Benjamini-Hochberg procedure for controlling the false discovery rate
  3. bMeans were separated using Fisher’s protected least significant difference (LSD) test at the 5% level of probability (from SPSS analysis)
  4. cP-value of < 0.05, 0.01, 0.001 is indicated by *, **, and ***, respectively (from SPSS analysis)