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Fig. 9 | BMC Plant Biology

Fig. 9

From: Tracing the biosynthetic origin of limonoids and their functional groups through stable isotope labeling and inhibition in neem tree (Azadirachta indica) cell suspension

Fig. 9

Expression profile and chemical inhibition of MVA and MEP pathway. (a) Effect of inhibitors, mevinolin and miconazole on growth of cells at different concentrations. (b) Effect of different concentrations of mevinolin on limonoid biosynthesis. (c)To study the involvement of cytochrome P450 enzymes in limonoid biosynthesis; miconazole an inhibitor of cytochrome enzymeswas used at different concentrations. (d) Chemically inhibited MVA and MEP pathway cells grown in the presence of [1,2-13C] Glc to monitor the formation of isotopologues. (e) Expression profile of HMGR and DXS genes in various tissues relative to the expression of HMGR2. (f) Effect of 0.1 mM fosmidomycin on MEP pathway metabolite, carotenoids. (g) control vs fosmidomycin treatment demonstrating the blanching of color of cell suspension due to drop in carotenoid level. (h) Comparison of expression profile of the studied genes in mevinolin treated cells with respect to the control

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BMC Plant Biology

ISSN: 1471-2229

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