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Fig. 6 | BMC Plant Biology

Fig. 6

From: Tracing the biosynthetic origin of limonoids and their functional groups through stable isotope labeling and inhibition in neem tree (Azadirachta indica) cell suspension

Fig. 6

Distribution of azadirachtin A isotopologues and isotopomers obtained from ESI-MS and MS/MS analysis. (a) Comparison of relative intensity of isotopologues of protonated ion adduct [M-H2O + H]+ of azadirachtin A obtained from independent labeling experiments with [1-13C], [2-13C], [1,6-13C] Glc. (b) Heatmap of isotopologue of azadirachtin A in the mass spectra vs relative intensity of each of the isotopologues for individual fragments (m/z 83.0498, 161.0597, 269.0808, 507.1650 derived from different part of the skeleton are represented) obtained when subjected to tandem MS at collision energy of 20%. The structure of the molecule and its fragments are given in grey boxes for the molecule denoting its 13C pattern of formation through MVA pathway and dashed line boxes for its formation through MEP pathway. The black dots and squares represent the position of 13C carbon incorporated from [1-13C] Glc / [1,6-13C] Glc whereas the red dots corresponding to those from [2-13C] Glc

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BMC Plant Biology

ISSN: 1471-2229

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