Fig. 5

The gcn20–1 mutant is sensitive to chemically induced DNA damage. a The wild type, gcn20–1 and GCN20::GCN20 gcn20–1 were grown with or without 0.01% MMS for 12 days before photographed. b Quantitative analysis of fresh weights of wild-type and gcn20–1 and GCN20::GCN20 gcn20–1 plants grown with or without 0.01% MMS for 12 days. c Relative fresh weights of wild-type, gcn20–1 and GCN20::GCN20 gcn20–1 plants treated with 0.01% MMS for 12 days compared with their untreated control. d The wild type and gcn20–1 and GCN20::GCN20 gcn20–1 were grown with or without 30 μM MMC for 12 days before photographed. e Quantitative analysis of survival rates of wild-type and gcn20–1 and GCN20::GCN20 gcn20–1 plants grown with or without 30 μM MMC treatment for 12 days. f Relative survival rates of wild-type, gcn20–1 and GCN20::GCN20 gcn20–1 plants treated with 30 μM MMC for 12 days compared with their untreated control. Seedlings were scored survival when plants had two or more true leaves. Data shown are means ± SEM. Asterisks indicate significant differences with respect to each control (Student’s t test): ***, P < 0.001