Fig. 4

DNA damage analysis of gcn20–1 mutant. a Analysis of DNA damage in wild-type, gcn20–1 and GCN20::GCN20 gcn20–1 plants by comet assay. b Quantitative analysis of the DNA in tails of wild-type, gcn20–1 and GCN20::GCN20 gcn20–1 plants in comet assay. c-e The expression of KU70 (c), KU80 (d) and RAD51 (e) was assayed by qPCR in the wild type, gcn20–1 and GCN20::GCN20 gcn20–1. The expression level of the wild type is set to 1 and ACT2 was used as an internal control. Data shown are means ± SEM. Asterisks indicate significant differences with respect to each control (Student’s t test): ***, P < 0.001