Fig. 7
From: Genome-wide analysis of the basic Helix-Loop-Helix (bHLH) transcription factor family in maize
Functional protein association network and Yeast two-hybrid assay. a Protein interaction network analysis revealed that most ZmbHLH proteins interact with more than one other bHLH protein. Line colors indicate the different kinds of evidence used to predict the network of protein interactions. b Yeast two-hybrid analysis of protein–protein interactions of various ZmbHLH proteins. AD and BD represent empty pGADT7 and pGBKT7 vectors, respectively. SD/-Leu/-Trp represents the synthetic dextrose media (SD) lacking Leu and Trp. SD/-Ade/-His/-Leu/-Trp/Aba indicates SD medium lacking Ade, His, Leu, and Trp but with Aureobasidin A