Table 4 Transformation and editing efficiencies
From: Efficient generation of stable, heritable gene edits in wheat using CRISPR/Cas9
Experiment | Construct | Genome target | No. of inoculated embryos | No. of transgenic lines | No. of edited lines (editing efficiency, %) | Embryo to edit efficiency |
|---|---|---|---|---|---|---|
GE1 | pRMH110 | A/B/D | 248 | 38 | 2 (5.3) | 0.8 |
GE2/4 | pRMH121 | B only | 157 | 28 | 0 (0) | – |
GE6 | pRMH120 | A only | 227 | 52 | 6 (11.5) | 2.6 |
GE7 | pRMH125 | D only | 213 | 35 | 4 (11.4) | 1.9 |
GE8 | pRMH123 | A only | 210 | 30 | 5 (16.7) | 2.4 |
GE11/12/13b | pRMH120 / pRMH125 | A and D | 432 | 73 | 13 (17.8) | 3 |
GE15/16 | pRMH131 | A and all three double | 223 | 27 | 4 (14.8) | 1.8 |
Hv6 | pRMH110 | N/A | 66 | 40 | 6 (15a) | 9 |