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Table 4 Transformation and editing efficiencies

From: Efficient generation of stable, heritable gene edits in wheat using CRISPR/Cas9

Experiment Construct Genome target No. of inoculated embryos No. of transgenic lines No. of edited lines (editing efficiency, %) Embryo to edit efficiency
GE1 pRMH110 A/B/D 248 38 2 (5.3) 0.8
GE2/4 pRMH121 B only 157 28 0 (0)
GE6 pRMH120 A only 227 52 6 (11.5) 2.6
GE7 pRMH125 D only 213 35 4 (11.4) 1.9
GE8 pRMH123 A only 210 30 5 (16.7) 2.4
GE11/12/13b pRMH120 / pRMH125 A and D 432 73 13 (17.8) 3
GE15/16 pRMH131 A and all three double 223 27 4 (14.8) 1.8
Hv6 pRMH110 N/A 66 40 6 (15a) 9
  1. GE Wheat experiments, Hv Barley experiment
  2. aHigh levels of somatic editing were observed making the true edit efficiency unclear
  3. bCo-transformation of two different Agrobacterium strains containing different constructs