From: Efficient generation of stable, heritable gene edits in wheat using CRISPR/Cas9
Vector | Genome targeted | Genome | Target sequence | Mismatches (bp) |
---|---|---|---|---|
pRMH110 | All genomes | gRNA | TTGTTTGCCAAGATTTTCCA | |
A | TTGTTTGCCAAGATTTTCCA | n/a | ||
B | TTGTTTGCCAAGATTTTCCA | n/a | ||
D | TTGTTTGCCAAGATTTTCCA | n/a | ||
Barley | TTGTTTGCCAAGATTTTCCA | n/a | ||
pRMH120 and pRMH123 | A genome | gRNA | CTGTGTATGAAGTTGTCCGG | |
A | CTGTGTATGAAGTTGTCCGG | n/a | ||
B | CTGCGTATGAAGTTGTCCAG | 2 | ||
D | CTGTGTATGAAGTTGCCCAG | 2 | ||
pRMH121 | B genome | gRNA | CTGCGTATGAAGTTGTCCAGC | |
A | CTGTGTATGAAGTTGTCCGGC | 2 | ||
B | CTGCGTATGAAGTTGTCCAGC | n/a | ||
D | CTGTGTATGAAGTTGCCCAGC | 2 | ||
pRMH125 | D genome | gRNA | GCCAGGGGAAGTCGAACTAA | |
A | GCCAAGGGA–––––––––––– | 11 | ||
B | GCCAAGGGA–––––––––––– | 11 | ||
D | GCCAGGGGAAGTCGAACTAA | n/a |