Nitric oxide contributes to methane-induced osmotic stress tolerance in mung bean

BMC Plant Biology

Table 1 Both CH₄- and NO-alleviated sugar metabolism inhibition caused by PEG stress were sensitive to PTIO

Treatments	Reducing sugar content (mg g^-1 DW)	Soluble sugar content (mg g^-1 DW)	α-amylase activity (mg min^-1g^-1 DW)	Amylase activity (mg min^-1g^-1 DW)
Con→Con	31.76 ± 1.80^b	18.60 ± 0.37^b	68.36 ± 5.16^b	429.37 ± 14.11^b
Con→PEG	20.71 ± 0.77^f	14.25 ± 0.77^f	21.28 ± 2.18^f	173.68 ± 4.19^f
CH₄→PEG	28.22 ± 0.45^d	16.77 ± 0.32^d	35.16 ± 3.25^d	275.48 ± 7.84^d
CH₄→Con	34.02 ± 0.78^a	21.11 ± 0.56^a	79.40 ± 5.05^a	474.73 ± 16.27^a
CH₄+PTIO→PEG	26.65 ± 0.47^e	15.85 ± 0.33^e	25.06 ± 2.83^e	219.84 ± 2.07^e
CH₄+PTIO→Con	30.87 ± 0.51^b	18.29 ± 0.28^b	63.14 ± 4.99^bc	412.25 ± 47.67^bc
SNP→PEG	28.67 ± 0.86^d	16.50 ± 0.82^d	38.82 ± 1.85^d	280.53 ± 10.74^d
SNP→Con	34.32 ± 1.88^a	21.32 ± 1.34^a	84.54 ± 3.93^a	488.89 ± 11.33^a
SNP+PTIO→PEG	26.18 ± 1.19^e	15.52 ± 0.05^e	26.30 ± 4.20^e	233.44 ± 7.07^e
SNP+PTIO→Con	30.37 ± 1.01^b	18.37 ± 0.93^b	62.00 ± 4.81^bc	412.83 ± 46.29^bc
PTIO→PEG	18.86 ± 0.99^g	12.74 ± 0.55^g	12.87 ± 3.01^g	127.62 ± 9.15^g
PTIO→Con	29.94 ± 0.59^c	17.28 ± 0.52^c	60.43 ± 0.50^c	393.47 ± 10.31^c

Mung bean seeds were presoaked with solutions containing 1.3 mM CH₄, 100 μM SNP, 500 μM PTIO, alone or their combinations for 12 h, and then shifted to 20% PEG-6000 for another 48 h. Afterwards, reducing and soluble sugar contents, α-amylase and total amylase activities in germinating seeds, were determined. Treatment with distilled water was regarded as control (Con). Within each set of experiments, data are means ± SE of three independent experiments with at least three replicates for each. Bars with different letters denote significant difference at P < 0.05 according to Duncan’s multiple test

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